{"id":49471,"date":"2025-06-10T09:52:41","date_gmt":"2025-06-10T09:52:41","guid":{"rendered":"https:\/\/photonics.laser2000.co.uk\/?post_type=product&p=49471"},"modified":"2025-12-17T14:36:07","modified_gmt":"2025-12-17T14:36:07","slug":"fast-controllable-led-illumination-for-fura-2-ratiometric-calcium-imaging","status":"publish","type":"product","link":"https:\/\/stagingphotonics.laser2000.co.uk\/?product=fast-controllable-led-illumination-for-fura-2-ratiometric-calcium-imaging","title":{"rendered":"Fast controllable LED illumination for Fura-2 ratiometric calcium imaging"},"content":{"rendered":"

The pE-340fura<\/sup> provides LED illumination for Fura-2 ratiometric calcium imaging.<\/strong><\/p>\n

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\"Print\"<\/p>\n

The 340nm and 380nm LED illumination system provides the optimum excitation wavelengths for Fura-2-based calcium imaging allowing high-precision, stable, high-throughput imaging with video-rate time resolution.<\/p>\n\n\n\n
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\"340nm
340 nm Excitation<\/figcaption><\/figure><\/td>\n
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\"380nm
380 nm Excitation<\/figcaption><\/figure><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

The images above show a field of cardiac myocytes (heart cells). The cells were loaded with Fura-2 using standard conditions (i.e. incubation with 2 micromolar Fura-2 acetoxymethyl ester for 30 minutes, followed by an additional 30 minutes for de-esterification.<\/p>\n

Images were obtained by Martin Bootman and Katja Rietdorf, School of Life, Health and Chemical Sciences, The Open University, UK.<\/em><\/p>\n

 <\/p>\n

Until recently, the response time of illumination systems for Fura-2 imaging has been limited to milliseconds due to mechanical switching of the wavelengths in arc lamp and monochromator systems. However, the pE-340fura<\/sup> can be digitally controlled using a TTL trigger for precise illumination control in as little as 20 microseconds.<\/p>\n

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 <\/p>\n

Spontaneous Ca2+ events are induced in Mg2+-free HBS. (A) Representative trace from a single hippocampal neuron of Mg2+-free induced Ca2+ events imaged at 0.5 Hz and (B) representative trace from two hippocampal neurons of Mg2+-free induced Ca2+ events imaged at 24.39 Hz.<\/p>\n

TINNING, P. W., FRANSSEN, A. J. P.M., HRIDI, S. U., BUSHELL, T. J. and MCCONNELL, G. (2017), A 340\/380 nm light-emitting diode illuminator for Fura-2 AM ratiometric Ca2+ imaging of live cells with better than 5 nM precision. Journal of Microscopy. doi:10.1111\/jmi.12616<\/em><\/p>\n

 <\/p>\n

Using the pE-340fura<\/sup> LED illumination system for Fura-2, less dye can be loaded into the cells whilst still maintaining the same measured calcium concentration and good signal-to-noise ratio. The reduction in required dye not only improves cell-viability due to reduced dye toxicity, but also results in a cost reduction per experiment.<\/p>\n

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Comparison of Ca2+ increases obtained from the application of trypsin (100 nM) to tsA-201 cells loaded with different concentrations of Fura-2 AM.<\/p>\n

TINNING, P. W., FRANSSEN, A. J. P.M., HRIDI, S. U., BUSHELL, T. J. and MCCONNELL, G. (2017), A 340\/380 nm light-emitting diode illuminator for Fura-2 AM ratiometric Ca2+ imaging of live cells with better than 5 nM precision. Journal of Microscopy. doi:10.1111\/jmi.12616<\/em><\/p>\n

There are two pE-340fura<\/sup> configuration options:<\/strong><\/p>\n

    \n
  • Direct-fit for connecting to microscopes \u2013 by selecting from a range of microscope adaptors which cover all current and most older models. A simple once only adjustment will allow optimisation to the optical path of the microscope.<\/li>\n
  • Liquid Light Guide with a fixed 3mm diameter, liquid light guide. An optional pE-340fura<\/sup> Universal Collimator can be specified in conjunction with a microscope adaptor if required, containing optics optimised to transmit the 340nm. CoolLED now include a light source stand for the pE-300fura<\/sup> illumination systems ordered with a liquid light guide (see picture 3 on the product pictures).<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"

    The pE-340fura is an LED illuminator for Fura-2 ratiometric calcium imaging, which also supports everyday fluorescence microscopy in a compact and affordable package.<\/p>\n","protected":false},"featured_media":49565,"template":"","meta":{"_acf_changed":false,"footnotes":""},"product_brand":[],"product_cat":[122],"product_tag":[],"class_list":{"0":"post-49471","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-led-illumination-systems","8":"first","9":"instock","10":"shipping-taxable","11":"product-type-simple"},"acf":[],"yoast_head":"\nLED illumination for Fura-2 Ratiometric Calcium Imaging - Laser 2000<\/title>\n<meta name=\"description\" content=\"pE-340fura ? 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